A retrospective clinicopathological study of inherited bleeding disorders in a tertiary care centre of Uttar Pradesh

 

Deepa Rani1, Pawan Pandey2, Anu Singh3*, Sandip Kumar4

1Associate Professor, Department of Pathology, IMS BHU, Varanasi India.

2Technical Assistant, Hematology Section, Department of Pathology, IMS BHU, Varanasi India.

3Assistant Professor, Department of Pathology, IMS BHU Varanasi India.

4Professor & Head, Department of Pathology, IMS BHU, Varanasi India.

*Corresponding Author E-mail: anusinghbangalore@gmail.com

 

ABSTRACT:

BACKGROUND: Inherited Bleeding Disorders (IBD) include various disease that reflect abnormalities of primary and secondary hemostasis. The pathophysiology of these disorders can be explained on the basis of vessel wall abnormalities, platelet disorders and coagulation factor defects. To further elaborate and enhance our understanding on these disorders, a 6-year retrospective study (2014-2020) was conducted on the patients referred to the coagulation section of the Hematology Department (Department of Pathology, IMS BHU). These included the ones who had suffered from bleeding tendencies at one or more sites with other relevant clinical history. AIM: - The objective of this study was to assess the prevalence, clinical spectrum, and haematological profile of inherited bleeding disorder among patients of Eastern UP and Bihar. It also focussed on various epidemiological factors including age, sex, family inheritance and consanguinity. MATERIALS AND METHODS: Three hundred and two patients matched our criteria. The age of these patients ranged from neonate to 50. A detailed relevant clinical history was taken for all the patients. These categories of patients were screened with routine tests like platelet count, Prothrombin Time (PT), Active Partial Thromboplastin Time (APTT), Breathing Time (BT), Clotting Time (CT) and a Complete Blood Cell Count (CBC). A factor assay was performed if indicated by the results of the screening assays. RESULTS: Out of 302 patients, 280patients (92.70%) were diagnosed with factor VIII deficiency. This category further comprised of 63.57% Hemophilia A cases (n=192), and 12.58%hemophilia B cases (n=38). Another cluster of 16.55% were diagnosed as Von Willebrand Disease (n=50). Also, a subset of the total patient population (7.30%) was diagnosed with an entity called Rare Inherited Coagulation Deficiency (RICD) which was further designated on the basis of specific factor assays. Most common clinical feature encountered was hematoma followed by ecchymosis, hemarthrosis, gum bleeding and epistaxis. CONCLUSION: The most common IBD was Hemophilia A in this subcategory of patients. Children under 5 year age were most affected making it the most vulnerable age group amounting to 38.73% of all recorded cases. Male population was more affected forming majority of the patients. Sporadic cases were more common than the inherited ones.

 

 

KEYWORDS: Bleeding disorders, Factor assays, Inherited, RICD.

 

 

 

INTRODUCTION:

Inherited bleeding disorder (IBD) includes various disease that reflect abnormalities of primary and secondary hemostasis. The pathophysiology of these disorders can be explained on the basis of vessel wall abnormalities, platelet disorders and coagulation factor defects. Disorders of primary hemostasis include vessel wall aberrations and platelet disorders (Qualitative and Quantitative). However, secondary hemostasis defects are associated with coagulation factor disarrays. The most common inherited bleeding disorders are Hemophilia A and B, which are relatively rare among other haematological abnormalities.1 In our study, we elaborated a new entity termed as RICD. To understand any pathology, first we need to know the normal physiology. After any trauma or injury to blood vessel, there is a spontaneous arrest of bleeding from the severed vessel by the process of hemostasis. The basic mechanisms are vascular constriction, formation of a platelet plug followed by clot formation produced by the constituents of blood at the site of injury. This hemostatic plug is formed within few minutes of trauma involving activation of the blood coagulation cascade.2,3 The final step is fibrous organization and clot retraction.

 

Any defect in the above mechanisms explained can lead to life-long bleeding disorders [4]. An elaborate clinical history of patient is required including duration, site and severity of previous episodes of bleeding. Also, requirements for blood transfusion, nature of the injury, reaction of hemostatic factors on exposure to previous haemostatic challenges (such as surgery, tooth extraction, childbirth) and presence of a family history if any is noted. Any drug history or nutritional deficiency should be excluded. A basic initial workup includes a complete blood count (CBC) with peripheral blood smear review, BT, CT, platelet count, prothrombin time (PT)/international normalised ratio (INR) and partial thromboplastin time (PTT), According to these findings, further investigations are warranted. In many cases, however, the routine use of a small battery of screening tests has merit because it usually saves time, and the results direct the course of further study.4

 

MATERIALS AND METHODS:

The study included patients with history of bleeding tendencies referred to the coagulation section of the Hematology Department (Department of Pathology, IMS BHU) from 2014 to 2020. An elaborate history of bleeding episodes including duration, site and severity was taken. Also, requirements for blood transfusion, nature of the injury, reactionof hemostatic factors on exposure to previous haemostatic challenges (such as surgery, tooth extraction, childbirth) was covered. Further, consanguinity, drug intake and family history of bleeding disorder were noted. Individuals who had a past history of intake of any drugs affecting platelet function in the previous four weeks were excluded from the study. None of these patients showed signs of acute Leukemia, Uraemia, Myelodysplastic syndrome, Myeloproliferative disorders, Dysproteinaemias, Cardiopulmonary bypass or any other secondary systemic disorders. A basic initial workup was done including complete blood count (CBC) with peripheral blood smear review, BT, CT, platelet count, prothrombin time (PT)/international normalised ratio (INR) and partial thromboplastin time (PTT), Based on the above derangements, individual coagulation factor assays were performed. All the tests of hemostasis were performed within 4 hours of blood collection. 2ml of venous blood was collected in EDTA vial for CBC tests and platelet counts. A peripheral blood smear was prepared from direct finger prick. After Leishman staining, slide was observed for any morphological abnormalities in platelets. For performing coagulation tests, Blood was collected in 3.2% sodium citrate vacutainer (blue cap) vial. One part of 3.2% sodium citrate buffer and nine parts of blood was taken (i.e. 0.1ml of anticoagulant for a 0.9ml specimen). Bleeding time (BT) was performed by Ivy’s modified template method, Additional tests performed were clot solubility test, PF3 availability and platelet aggregation tests. For performing platelet studies, platelet rich plasma was prepared and coagulation studies were done on platelet poor plasma. Platelet-rich plasma was prepared by 600 rpm for 3 minutes while platelet-poor plasma was prepared by 2,500 rpm for 10 minutes for the coagulation profile Platelet aggregation test with adenosine diphosphate (ADP) and adrenaline was done to detect platelet function defects. Both semi-automated and fully automated Stago equipment and reagents were used for factor assays. Correction experiment was also performed to know the specific factor deficiency or inhibitors present, with normal plasma, normal-aged serum, and Al(OH)3 adsorbed plasma. On the basis of these assay, specific factor assay was performed further. If all the coagulation tests were normal, then factor XIII assay (urea solubility test) was performed. Test for vWD detected Von Willebrand factor antigen (vWFAg) by enzyme-linked immunosorbent assay (Diagnostica Stago, France),.

 

OBSERVATION AND RESULTS

 

Table 1: Prevalence (%) of inherited bleeding disorder in the present study (n=302)

Diagnosis

Frequency

Percent

Hemophilia –A

192

63.57

Hemophilia –B

38

12.58

Vonwillebrand

50

16.55

Platelet Disorder

13

4.30

Factor XIII

6

1.98

Factor X

2

0.66

Factor VII

1

0.33

Total

302

100.00

 

Table 2: Severity

Severity

Frequency

Percent

Severe

191

63.24

Moderate

55

18.21

Mild

56

18.54

Total

302

100.00

Table 3: Age distribution

Age

Frequency

Percent

<1

25

8.27

1-5

92

30.46

6-10

47

15.56

11-20

92

30.46

21-30

27

8.94

>31

19

6.29

Total

302

100.00

 

Table 4: Sex distribution

SEX

Frequency

Percent

Male

253

83.77

Female

49

16.22

Total

302

100.00

 

Table 5: Inheritance

FAMILY HISTORY

Frequency

Percent

Present

122

40.39

Absent

180

59.60

Total

302

100.00

 

Table 6: Consanguinity

Consanguinous

Frequency

Percent

Present

22

7.28

Absent

280

92.71

Total

302

100.00

 

Table 8: District via distribution of inherited bleeding disorder

District

Frequency

Percent

Allahabad

5

1.65

Azamgarh

23

7.61

Ballia

23

7.61

Basti

6

1.98

Bhadohi

20

6.62

Chandauli

22

7.28

Deoria

13

4.30

Ghazipur

19

6.29

Gorakhpur

2

0.66

Jaunpur

30

9.93

Kaushambi

1

0.33

Kushinagar

12

3.97

Maharajgunj

12

3.97

Mau

34

11.25

Mirzapur

21

6.95

Sonbhadra

14

4.63

Sultanpur

3

0.99

Varanasi

42

13.90

Total

302

100.00

Varanasi 13.90%, Mau 11.25%, Jaunpur 9.93%, being higher prevalence area while Gorakhpur, Kaushambi, Sultanpurstaying<1%.

 

Table 7: Clinical Presentation

Clinical Presentation

Present

Absent

No.

%

No.

%

Umblical cord bleeding

6

1.98

296

98.01

Purpura

11

3.64

291

96.35

Epistaxis

75

24.83

227

75.16

Petechiae

23

7.61

279

92.38

Ecchymosis

158

52.31

144

47.68

Epistaxis

75

24.83

227

75.16

Malena

33

10.92

269

89.07

Hematemesis

8

2.64

294

97.35

Hematuria

22

7.28

280

92.71

Hemarthrosis

158

52.31

144

47.68

Haematoma

201

66.55

101

33.44

Menorrhagia

31

10.26

271

89.73

Gum Bleeding

78

25.82

224

74.17

 

RESULTS: 

On the basis of clinical history and screening parameters, three hundred and two individual patients were entitled for the study. After detailed analysis, these patients were labelled under the broad spectrum of inherited bleeding disorders. As mentioned in Table 1, Out of 302 patients, 280patients (92.70%) were diagnosed with factor VIII deficiency. These category further comprised of 63.57% Hemophilia A cases (n=192), and 12.58% Hemophilia B cases (n=38). Another cluster of 16.55% were diagnosed as vWD (n=50). A subset of the total patient population (7.30%) were diagnosed with an entity called Rare Inherited Coagulation Deficiency (RICD). Among these, Platelet function disorders was found in 13 individuals amounting for 4.30% of the total percentage, Factor XIII deficiency was in 6 (1.98%), Factor X deficiency in 2 (0.66%) and  Factor VII deficiency in 1 (0.33%). The proportion of Hemophilia A: B was 5:1. Majority of the cases showed severe signs and symptoms (63.24%) of all the cases (Table 2). Delayed presentation and diagnosis was a common feature. Children under 5 year age were most affected making it the most vulnerable age group amounting to 38.73% of all recorded cases (Table 3). After analysing sex incidence, it was observed that males had higher frequency of coagulation factor defects than females, frequency being 83.77 percent (Table 4). 40.39% of patients had family history of bleeding manifestations.(Table 5). As mentioned in Table 6, consanguinity was present in minor percentage (7.28%) The most common presenting feature was hematoma (66.55%) followed by ecchymosis (52.31%) and hemarthrosis (52.31%) jointly (Table 7). Hallmark feature of umbilical cord bleeding was reported in patients with factor XIII deficiency (1.98%). (Table 7). We also tabulated (Table 8) district wise distribution of inherited bleeding disorders by analysing individual records of each patient. However, this interpretation was biased due to lack of awareness and accessibility among patients and ignorance of health services in few districts.

 

DISCUSSION:

In the current study, Hemophilia was most prevalent bleeding disorder followed by vWD. Incidence of Hemophilia A is approximately 1 in 10000 people and it is five times more common than Hemophilia B. [4] Similar findings were noted in our study. HA and HB are X linked diseases and genes for HA is located in the tip of the long arm of X chromosomes in band Xq28 and for HB in band Xq27. Majority of severe HA have large deletions or inversions (intron 22, intron 1) leading to major defects of factor VIII.4  Factor VIII (hemophilia A), Factor IX (hemophilia B), and von Willebrand's factor deficiency are the most common inherited coagulation defects. Both hemophilia A (HA) and hemophilia B (HB) show X-linked recessive inheritance. Factor XI deficiency also called hemophilia C is rarer, milder, and autosomally inherited.5 In about 59.60% of the total 302 patients in the current study, there was no family history, could have been due to spontaneous mutations or transfers by mildly affected or carrier parents who were asymptomatic and undiagnosed.The current study data suggests the scattered nature of disease as a result of spontaneous genetic mutation. Nearly 2/3rd of all Hemophilia who underwent factor assay had acute disease suggestive of prevalence of severe Hemophilia in the North Indian population, although referral bias due to past presentation of severe cases to health care facility could be another possible ground. Few studies have been carried out in India to show the prevalence of bleeding disorders associated with factor deficiencies and their clinical manifestations.6-11 this particular study was carried out to help best define the frequency of these bleeding disorders in this area of the world. Similar to available literature from Indian subcontinent 6-10 as well as Middle East Asia11 and Pakistan12,13, our study showed Hemophilia A to be the commonest bleeding disorder. A large number of patients in our country with inherited bleeding disorders remain undiagnosed because of the limitation of coagulation laboratories and lack of awareness among health care professionals and family members. Henceforth, the figures presented in this study do not reflect the actual burden of the bleeding disorders in our community. 14

 

In current study, the incidence of PFD and vWD was found to be around 4.30% and 16.55% respectively as compared to the previous studies which reported incidence of PFD and vWD from 12.8 to 39.4% 8, 13 and 6.5 to 20% 11, 15 respectively. Also, a recent study [16] showed incidence as 7.5% and 4.4% respectively. Plausible explanation for dipping incidence could be that PFD and vWD are autosomal disorders and have been caught to have higher incidence in communities where consanguinity is customary. The consanguinity in our evaluated population was low (7.28%) in contrast to national level study which was (10.6%)17. Common sites of bleeding were mucocutaneous and hemathrosis. The low numbers of patients getting replacement treatment is because of underreporting and may not be the real depiction of the consumption of health resources at our institute and as well of the resources used by the bleeding diathesis patients in their life time, as most of these patients were advised to be followed up at local public health centres for minor bleeds and replacement therapy. Therapy with fresh frozen plasma is the most frequently used therapy modality with factor replacement therapy used only in a smaller fraction. Majority of the patients were lost to follow up after first visit.

 

CONCLUSION: 

In our findings, prevalence of inherited bleeding disorder were as follows, 63.57% through Hemophilia A , 12.58% via Hemophilia B, Factor XIII deficiency 1.98%, Factor X deficiency 0.66% and a single case of factor VII deficiency 0.33%. vWD was present in 16.55% of all cases while Platelet function disease was spotted in 4.30% cases. Most of the recorded cases in the study resulted due to sporadic mutations. Children under 5 years of age were most affected making it the most vulnerable age group amounting to 38.73% of all recorded cases. There is a need to keep a check on bleeding manifestations in childhood and should be attended carefully on time. Connection between an individual and its family member is rare and happens only in emergencies due to migration for work. Due to less number of coagulation laboratories and lack of knowledge among healthcare practitioners and family members, a substantial number of patients with inherited bleeding disorders go undetected and thus the data presented in this study do not represent the actual burden of the disease condition in our state. These patients receive diagnostic and therapeutic care through NGOs and get very little assistance from the establishment. The administration should ensure adequate infrastructure for detection as well as funding for the treatment and management of these patients so that more and more patients across the state live well.

 

CONFLICTS OF INTEREST:

There are no conflicts of interest.

 

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Received on 09.07.2022            Modified on 19.08.2022

Accepted on 13.09.2022           © RJPT All right reserved

Research J. Pharm. and Tech 2022; 15(10):4772-4776.

DOI: 10.52711/0974-360X.2022.00801